This is a knockout-validated antibody summary, based on the publication "Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. To verify the specificity of the fgl2 antibody, the expression of fgl2 in the livers of MCD-fed or HFD-fed WT and fgl2-/- mice was detected by immunohistochemistry (A, B). From [1].

Mouse monoclonal IgG2a Kappa

Company: Abnova

Antibody: FGL2

Catalog number: H00010875-M01

Summary: Mouse monoclonal antibody raised against a partial recombinant FGL2. Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin), immunoprecipitation, and ELISA.

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Figure 2. To verify the specificity of the fgl2 antibody, the expression of fgl2 in the livers of MCD-fed or HFD-fed WT and fgl2-/- mice was detected by western blotting. From [1].

Immunohistochemistry | Western blot

WT and fgl2-/- mouse livers.

WB: 5% milk.

WB: 1:1,000 dilution.

IHC: 1:100 dilution.

WB: 1:5,000 dilution HRP goat anti-mouse IgG (A21010, Abbkine, USA).

WB: Enhanced chemiluminescence system (ChemiDoc XRS+, Bio-Rad, USA).

The same clone (6D9) is sold as MilliporeSigma WH0010875M1; LifeSpan Biosciences LS-C105107; Abnova H00010875-M01.