This is a knockout-validated antibody summary, based on the publication "The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence of FOP and CP170 in control and FOP KO RPE-hTERT cells. Scale bar, 10 um. Courtesy from the Authors [1].

Mouse monoclonal IgG2b Kappa

Company: Novus Biologicals

Antibody: FGFR1OP

Catalog number: H00011116-M01

Summary: Mouse monoclonal IgG2b Kappa against full length recombinant FGFR1OP (FOP) protein. Reacts with human by western blot, immunocytochemistry/immunofluorescence and ELISA.

Immunocytochemistry

Control and FOP KO RPE-hTERT cells..

5% normal donkey serum (017-000-121, Jackson ImmunoResearch) in immunofluorescence (IF) buffer (3% BSA and 0.1% NP-40 in PBS) for 30 min at room temperature.

1:1,000 dilution in IF buffer for at least 2 hr at room temperature.

Fluorescent-labeled secondary antibody (Jackson ImmunoResearch) diluted in IF buffer for 1 hr at room temperature.

Everest deconvolution workstation (Intelligent Imaging Innovations) equipped with a Zeiss AxioImager Z1 microscope and a CoolSnapHQ cooled CCD camera (Roper Scientific). A 63x NA1.4 Plan-Apochromat objective lens (420780-9900, Zeiss) was used.

The same clone (2B1) is sold as Abnova H00011116-M01; Sigma-Aldrich WH0011116M1.