FBXO7 antibody | knockout validation | Santa Cruz sc-271763
DOI
//dx.doi.org/10.13070/ko.en.7.2119
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "Loss of FBXO7 (PARK15) results in reduced proteasome activity and models a parkinsonism-like phenotype in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: FBXO7

Catalog number: sc-271763

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 427-461 within an internal region of human FBXO7. Recommended for detection of FBXO7 of human, mouse and rat origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Brain lysates from P5 FBXO7+/+, FBXO7+/-, or FBXO7-/- mice.

Secondary incubation

1:1,000 dilution HRP-coupled goat anti-mouse (Dianova, 11-035- 146)

Figure

Please see Figure 1c in the article [1].

References
  1. Vingill S, Brockelt D, Lancelin C, Tatenhorst L, Dontcheva G, Preisinger C, et al. Loss of FBXO7 (PARK15) results in reduced proteasome activity and models a parkinsonism-like phenotype in mice. EMBO J. 2016;35:2008-25 pubmed publisher