FBXO6 antibody | knockout validation | Santa Cruz sc-134339

This is a knockout-validated antibody summary, based on the publication "FBXO6-mediated RNASET2 ubiquitination and degradation governs the development of ovarian cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

FBXO6 antibody | knockout validation | Santa Cruz sc-134339 figure 1
Figure 1. A2780 FBXO6 knockout (FBXO6−/−) cells were generated by CRISPR assay and the WCLs were subjected to western blot analysis with indicated antibodies. From [1].
Antibody information

Mouse monoclonal IgG1κ

Company: Santa Cruz

Antibody: FBXO6

Catalog number: sc-134339

Summary: Mouse monoclonal antibody against recombinant human FBXO6 protein. Recommended for detection of FBXO6 of human origin by western blot, immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

Control and FBXO6 knockout A2780 cells.

Blocking agent

5% non-fat milk in phosphate-buffered saline (PBS) for 1h.

Primary incubation

1:1,000 dilution overnight at 4°C.

Secondary incubation

For 1h at room temperature.

References
  1. Ji M, Zhao Z, Li Y, Xu P, Shi J, Li Z, et al. FBXO6-mediated RNASET2 ubiquitination and degradation governs the development of ovarian cancer. Cell Death Dis. 2021;12:317 pubmed publisher