FANCD2 antibody | knockout validation

FANCD2 antibody | knockout validation | Santa Cruz sc-20022

This is a knockout-validated antibody summary, based on the publication "Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis showing the expression levels of FANCD2 and UHRF1 in the cell lines used. From [1].

Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: FANCD2

Catalog number: sc-20022

Summary: Mouse monoclonal IgG1 κ against the N-terminus of human FANCD2. Recommended for detection of S and L isoforms of FANCD2 of human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

WT and FANCD2 -/- HeLa cells.

Primary incubation

1:200 dilution.

Clone note

The same clone (FI17) is sold as Novus Biologicals NB100-316; OriGene TA309656; Santa Cruz Biotechnology sc-20022; LifeSpan Biosciences LS-B201; Invitrogen MA1-16570.

References

Lopez Martinez D, Kupculak M, Yang D, Yoshikawa Y, Liang C, Wu R, et al. Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage. Cell Rep. 2019;27:2990-3005.e5 pubmed publisher

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