FANCC antibody | knockout validation | Millipore MABC524

This is a knockout-validated antibody summary, based on the publication "Validating the concept of mutational signatures with isogenic cell models", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

FANCC antibody | knockout validation | Millipore MABC524 figure 1
Figure 1. Immunoblots for expression of the indicated proteins in CRISPR-Cas9 edited human HAP1 clones. Actin serves as a loading control. “*” denotes a non-specific band. From [1].
Antibody information

Mouse monoclonal IgG2bκ

Company: Millipore

Antibody: FANCC

Catalog number: MABC524

Summary: Mouse monoclonal IgG2bκ against a linear peptide corresponding to human FANCC. Reacts with rat, mouse and human. Suitable for western blot, immunoprecipitation and immunocytochemistry.

Validation Method

Western blot

Sample

Control and FANCC-KO human HAP1 cells. Cell extracts were prepared using RIPA lysis buffer (NEB) with protease (Sigma) and phosphatase (Sigma) inhibitors.

Primary incubation

1:500 dilution.

Clone note

The same clone (8F3) is sold as LifeSpan Biosciences LS-C142660, LS-C142661, LS-C142662, LS-C142664; EMD Millipore MABC524.

References
  1. Zou X, Owusu M, Harris R, Jackson S, Loizou J, Nik Zainal S. Validating the concept of mutational signatures with isogenic cell models. Nat Commun. 2018;9:1744 pubmed publisher