FAM50A/XAP5 antibody | knockout validation

FAM50A/XAP5 antibody | knockout validation | Abcam ab186410

This is a knockout-validated antibody summary, based on the publication "Combinatorial CRISPR screen identifies fitness effects of gene paralogues", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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FAM50A/XAP5 antibody | knockout validation | Abcam ab186410 figure 1

Figure 1. Western blot confirming knockout of FAM50A in three clones. Two FAM50B knockout clones are shown as controls. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: FAM50A/XAP5

Catalog number: ab186410

Summary: Rabbit monoclonal antibody against a synthetic peptide of FAM50A/XAP5. Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry/immunofluorescence and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and FAM50A KO A375 cell lines.

Primary incubation

1:3,000 dilution.

References

Thompson N, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, et al. Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Nat Commun. 2021;12:1302 pubmed publisher

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