This is a knockout-validated antibody summary, based on the publication "A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Single-cell FAK-KO cells were generated by CRISPR-Cas9. Protein expression of FAK, p-paxillin (Y118), and paxillin in control and FAK gene deleted (FAK-KO) Ocy454 cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: FAK

Catalog number: 13009

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ser893 of human FAK protein. Reacts with human and mouse. Suitable for western blot and immunoprecipitation.

Western blot

Control and FAK-KO Ocy454 cells.

5% milk in tris-buffered saline plus 0.05% Tween-20 (TBST).

1:1,000 dilution overnight at 4 °C

HRP-coupled secondary antibodies.

ECL Western Blotting Substrate (Pierce), ECL Plus Western Blotting Substrate (Pierce), or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo scientific).