This is a knockout-validated antibody summary, based on the publication "Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting of the indicated proteins WT and FADD knockout HeLa cells. From [1].

Mouse monoclonal IgG1

Company: BD Transduction

Antibody: FADD

Catalog number: 610400

Summary: Mouse monoclonal IgG1 against human FADD (aa. 94-208). Reacts with human by western blot and immunofluorescence. Not recommended for immunohistochemistry or immunoprecipitation.

Western blot

WT and FADD KO HeLa cells. Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche).

5% nonfat dry milk in Tween–Tris-buffered saline (TBS) (TTBS) for 1 h at room temperature.

1:1,000 dilution in blocking solution for 1 to 2 h at room temperature or overnight at 4°C with agitation.

1:5,000 dilution HRP-conjugated anti-mouse (Zymax) in blocking buffer for 1 h at room temperature.

Enhanced chemiluminescence (ECL) reaction using a freshly prepared ECL reagent (Promega).