Exo84 antibody | knockout validation | Santa Cruz sc-515532

This is a knockout-validated antibody summary, based on the publication "Genome-scale CRISPR screening for modifiers of cellular LDL uptake", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Exo84 antibody | knockout validation | Santa Cruz sc-515532 figure 1
Figure 1. Immunoblotting of lysates prepared from wild-type HuH7 cells or EXOC8-4/+2 clones with and without ectopic lentiviral expression of EXOC8 CRISPR-resistant cDNA. From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Exo84

Catalog number: sc-515532

Summary: Mouse monoclonal antibody against amino acids 564-725 mapping near the C-terminus of human Exo84 (EXOC8). Recommended for detection of Exo84 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and EXOC8-KO HuH7 cells.

Primary incubation

1:500 dilution.

References
  1. Emmer B, Sherman E, Lascuna P, Graham S, Willer C, Ginsburg D. Genome-scale CRISPR screening for modifiers of cellular LDL uptake. PLoS Genet. 2021;17:e1009285 pubmed publisher