This is a knockout-validated antibody summary, based on the publication "A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot analysis of ovarian tissue extracts from ERβ KO, Y55F KI, and WT littermates. From [1].

Mouse monoclonal IgG2b

Company: DSHB

Antibody: Estrogen receptor beta (ER 2)

Catalog number: CWK-F12

Summary: Mouse monoclonal antibody against E2 ligand-binding domain of human Estrogen receptor beta (ER 2). Reacts with human, mouse, porcine, and rat. Suitable for western blot, immunoprecipitation (IP), immunofluorescence, immunohistochemistry, Chromatin IP, ELISA, FFPE, and function blocking,

Western blot

WT and ERβ KO mouse ovarian tissues.

HRP-conjugated secondary antibodies.

Immunoblotting signals were visualized with ECL Western Blotting Substrate (Thermo Fisher).