This is a knockout-validated antibody summary, based on the publication "A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot analysis of ovarian tissue extracts from ERβ KO, Y55F KI, and WT littermates. From [1].

Mouse monoclonal IgG2b

Company: Thermo Fisher

Antibody: Estrogen Receptor Beta

Catalog number: MA5-24807

Summary: Mouse monoclonal antibody against a synthetic peptide of human Estrogen Receptor beta (aa 2-88). Reacts with human. Suitable for western blot, immunoprecipitation, immunohistochemistry, and ELISA.

Western blot

WT and ERβ KO mouse ovarian tissues.

HRP-conjugated secondary antibodies.

Immunoblotting signals were visualized with ECL Western Blotting Substrate (Thermo Fisher).

The same clone (PPZ0506) is sold as R&D Systems PP-PPZ0506-00; Invitrogen MA5-24807.