Esrp1/2 antibody | knockout validation | Rockland Immunochemicals 210-301-C31
DOI
//dx.doi.org/10.13070/ko.en.7.2200
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Esrp1/2 antibody | knockout validation | Rockland Immunochemicals 210-301-C31 figure 1
Figure 1. Western blot confirmation of Esrp1 and Esrp2 KO in purified epidermis from E18.5 embryos (n = 2). From [1].
Antibody information

Mouse monoclonal IgG1 or IgG2b,kappa.

Company: Rockland Immunochemicals

Antibody: Esrp1/2

Catalog number: 210-301-C31

Summary: Mouse monoclonal IgG1 or IgG2b,kappa against full length recombinant mouse Esrp-1 fusion protein. Reacts with mouse by western blot and ELISA.

Validation Method

Western blot

Sample

Protein lysate from total epidermis from mouse embryos with Esrp1 and Esrp2 combined deletions.

Blocking agent

5% non-fat milk in PBST.

Primary incubation

1:1000 dilution overnight at 4°C.

Secondary incubation

Sheep anti-mIgG:HRP at 1:2500 (GE Healthcare).

Detection

ECL detection (Invitrogen) by chemiluminescence.

Clone note

The same clone (23A7.C9) is sold as OriGene TA319605; Abnova MAB10272; Rockland Immunochemicals 210-301-C31.

References
  1. Bebee T, Park J, Sheridan K, Warzecha C, Cieply B, Rohacek A, et al. The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development. elife. 2015;4: pubmed publisher