EphB2 antibody | knockout validation | ThermoFisher 37-1700

This is a knockout-validated antibody summary, based on the publication "A T cell redirection platform for co-targeting dual antigens on solid tumors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

EphB2 antibody | knockout validation | ThermoFisher 37-1700 figure 1
Figure 1. Western blot of KP4 parental cells and clonal lines established by CRISPR/Cas9: EPHB2 KO and un-edited WT cell line. From [1].
Antibody information

Mouse monoclonal IgG1, kappa

Company: ThermoFisher

Antibody: EphB2

Catalog number: 37-1700

Summary: Mouse monoclonal antibody against a synthetic peptide derived from the C-terminal region of the recombinant EphB2 protein. Reacts with human and chicken. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, and ELISA.

Validation Method

Western blot

Sample

Control and EPHB2-KO KP4 cells.

Blocking agent

5% skim milk.

Secondary incubation

Anti-mouse-HRP (CellSignaling #7076)

References
  1. Enderle L, Shalaby K, Gorelik M, Weiss A, Blazer L, Paduch M, et al. A T cell redirection platform for co-targeting dual antigens on solid tumors. MAbs. 2021;13:1933690 pubmed publisher