This is a knockout-validated antibody summary, based on the publication "A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: EphB2

Catalog number: sc-130068

Summary: Mouse monoclonal IgG2b (kappa light chain) against a recombinant protein corresponding to amino acids 17-200 of human EphB2. Recommended for detection of EphB2 of mouse, rat and human by western blot, immunoprecipitation, immunofluorescence and ELISA

Western blot

WT and EPHB2 KO Caki-1 cells. Cells were harvested by scraping in PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer (150 mM sodium chloride, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris, and a protease inhibitor cocktail [Roche] ).

3% BSA and 10% FBS in TBST (20 mM Tris, 150 mM sodium chloride, 0.1% Tween 20).

Diluted in blocking buffer.

IRDye 800CW and 680RD secondary antibodies from Li-Cor Biosciences.

Li-Cor Odyssey infrared scanner and analyzed in ImageStudio Lite 5.2 (Li-Cor Biosciences).

Immunoblot of EphB2 in WT and EPHB2 KO Caki-1 cells. GAPDH was used as a loading control. Please see Figure 6f in the article [1].

The same clone (2D12C6) is sold as Santa Cruz Biotechnology sc-130068; LifeSpan Biosciences LS-B3904; Abnova MAB10592; Invitrogen MA5-15397; ProMab 20199; EMD Millipore MABN726.