EphB2 antibody | knockout validation | Santa Cruz sc-130068

This is a knockout-validated antibody summary, based on the publication "A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: EphB2

Catalog number: sc-130068

Summary: Mouse monoclonal IgG2b (kappa light chain) against a recombinant protein corresponding to amino acids 17-200 of human EphB2. Recommended for detection of EphB2 of mouse, rat and human by western blot, immunoprecipitation, immunofluorescence and ELISA

Validation Method

Western blot

Sample

WT and EPHB2 KO Caki-1 cells. Cells were harvested by scraping in PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer (150 mM sodium chloride, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris, and a protease inhibitor cocktail [Roche] ).

Blocking agent

3% BSA and 10% FBS in TBST (20 mM Tris, 150 mM sodium chloride, 0.1% Tween 20).

Primary incubation

Diluted in blocking buffer.

Secondary incubation

IRDye 800CW and 680RD secondary antibodies from Li-Cor Biosciences.

Detection

Li-Cor Odyssey infrared scanner and analyzed in ImageStudio Lite 5.2 (Li-Cor Biosciences).

Figure

Immunoblot of EphB2 in WT and EPHB2 KO Caki-1 cells. GAPDH was used as a loading control. Please see Figure 6f in the article [1].

Clone note

The same clone (2D12C6) is sold as Santa Cruz Biotechnology sc-130068; LifeSpan Biosciences LS-B3904; Abnova MAB10592; Invitrogen MA5-15397; ProMab 20199; EMD Millipore MABN726.

References
  1. TerBush A, Hafkamp F, Lee H, Coscoy L. A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5. J Virol. 2018;92: pubmed publisher