EphA2 antibody | knockout validation | Cell Signaling 6997

This is a knockout-validated antibody summary, based on the publications "Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry", as cited below [1], and "A T cell redirection platform for co-targeting dual antigens on solid tumors" for western blot knockout validation (figure s6b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

EphA2 antibody | knockout validation | Cell Signaling 6997 figure 1
Figure 1. Knockout efficiency of EphA2 in nasopharyngeal epithelial cells TW03. β-Actin was used as the loading control. Courtesy from the Authors [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: EphA2

Catalog number: 6997

Summary: Rabbit monoclonal IgG against a human EphA2 recombinant protein fragment. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

Control and EphA2-knockout AGS cell lines. Cells were lysed in RIPA buffer containing a protease inhibitor mixture (Roche).

Secondary incubation

Horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (#31460, Invitrogen).

Detection

Enhanced chemiluminescence (ECL, Millipore).

References
  1. Zhang H, Li Y, Wang H, Zhang A, Chen M, Fang Z, et al. Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. Nat Microbiol. 2018;3:1-8 pubmed publisher
  2. Enderle L, Shalaby K, Gorelik M, Weiss A, Blazer L, Paduch M, et al. A T cell redirection platform for co-targeting dual antigens on solid tumors. MAbs. 2021;13:1933690 pubmed publisher