This is a knockout-validated antibody summary, based on the publication "EBF2 promotes the recruitment of beige adipocytes in white adipose tissue", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Sheep polyclonal IgG
Company: R & D Systems
Antibody: EBF-2
Catalog number: AF7006
Summary: Sheep polyclonal raised against a recombinantly expressed (E. coli) polypeptide corresponding to residues 407-519 of mouse EBF-2.
Antibody detects mouse and human EBF-2.
Supplier recommended for Western blot and IHC.
Immunohistochemistry
White adipose tissue cells from wild-type and ERB-2 -/- mice.
Cells were treated with 4% paraformaldehyde overnight, washed with PBS and then incubated overnight with PBS containing 5% Donkey serum and 0.5% Triton X-100.
PBS containing 5% Donkey serum and 0.5% Triton X-100.
Overnight in blocking buffer. Antibody used at 1:200 dilution.
Secondary antibody (Alexa Fluor conjugated, Life Technologies) incubated in blocking buffer for 2 hours at room temperature.
Leica TCS Sp8 confocal microscope.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
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