This is a knockout-validated antibody summary, based on the publication "A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot data showing EZH2 protein expression in RD cells at 15 and 20 days post-infection with a lentiviral vector encoding Cas9 and each of the three independent sgRNAs targeting EZH2. From [1].

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: EZH2

Catalog number: 3147

Summary: Mouse monoclonal IgG1 against a recombinant protein containing human Ezh2 residues 351-453. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunofluorescence/immunocytochemistry.

Western blot

Cells were lysed in RIPA buffer containing 10 % SDS and Complete Protease Inhibitor Cocktail Tablets (Roche).

Fluorescent imaging using the Odyssey infrared imaging system (LI-COR Biosciences; Millennium Science, Surrey Hills, Australia).

The same clone (AC22) is sold as Active Motif 39875; eBioscience 14-9867-80, 14-9867-82, 50-9867-82; Cell Signaling Technology 3147; EMD Millipore 17-662.