EZH2 antibody | knockout validation | BD Biosciences 612667

This is a knockout-validated antibody summary, based on the publication "A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

EZH2 antibody | knockout validation | BD Biosciences 612667 figure 1
Figure 1. Immunofluorescence analysis of biotinylated GNA (Bio-GNA) and EZH2 in the nuclei of mouse embryonic fibroblasts (MEFs). Scale bar, 25 um. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: EZH2

Catalog number: 612667

Summary: Mouse monoclonal IgG1 against human EZH2 recombinant protein aa. 156-256. Reacts with human, mouse, rat, dog, and chicken. Suitable for western blot and immunofluorescence.

Validation Method

Immunocytochemistry

Sample

EZH2+/+ and EZH2-/- mouse embryonic fibroblasts (MEFs). Cells were fixed with 4% paraformaldehyde.

Blocking agent

5% bovine serum albumin.

Primary incubation

For 2 h at 37°C.

Secondary incubation

Fluorescein isothiocyanate (FITC)-conjugated secondary antibody (ZYMED, San Francisco, CA, USA) for 2 h at 37°C.

Detection

Laser confocal microscope (TCS SP2, Leica, Germany).

References
  1. Wang X, Cao W, Zhang J, Yan M, Xu Q, Wu X, et al. A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.. EMBO J. 2017;36:1243-1260 pubmed publisher