This is a knockout-validated antibody summary, based on the publication "Uvrag targeting by Mir125a and Mir351 modulates autophagy associated with Ewsr1 deficiency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: EWSR1

Catalog number: sc-28327

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 2-19 within an internal region of EWS of human origin. Recommended for detection of EWS of human , mouse and rat. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and canine, bovine, porcine and avian.

Western blot | Immunocytochemistry

WT and ewsr1-/- MEFs. WB: Cells were lysed in buffer (10 mM Tris at pH 7.4, 1 mM ethylenediaminetetraacetic acid EDTA, pH 8.0, 500 mM NaCl, and 0.5% Triton X-100 Amresco, 0694).

1:250 dilution

WB: horseradish peroxidase secondary (Pierce, 170-6516).

WB: ImmobilonTM Western ECL solution (Millipore, 34096) and a Kodak Image Station 4000MM (Kodak, 745280) were employed.

ICC: spinning disk confocal microscope (Olympus DSU, Tokyo, Japan).

Please see Figure 1a,d and Figure 2d in the article [1].

Presumably the same antibody was used for ICC. The feedback from the Authors is sought, and this summary will be updated upon any reply.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.