ERbeta antibody | knockout validation | Zymed (Thermo Fisher) 51-7700
DOI
//dx.doi.org/10.13070/ko.en.6.1870
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Plasma membrane estrogen receptors exist and functions as dimers", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal

Company: Zymed (Thermo Fisher Scientific)

Antibody: ERbeta

Catalog number: 51-7700

Summary: Rabbit polyclonal antibody raised against an 19 amino acid synthetic peptide corresponding to a region in the C-terminus of human ERbeta. Supplier recommended for flow cytometry, IHC and WB. Antigen affinity-purified. Reacts with human, dog, fish and mouse ERbeta.

Validation Method

Western blot

Sample

Lysates of wild-type and ERbeta knockout mouse brain capillary endothelial cells (MBCEC).

Primary incubation

Anti-ERbeta antibody.

Figure

See Figure 1 in [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Razandi M, Pedram A, Merchenthaler I, Greene G, Levin E. Plasma membrane estrogen receptors exist and functions as dimers. Mol Endocrinol. 2004;18:2854-65 pubmed