This is a knockout-validated antibody summary, based on the publications "Inverse Relationship between Progesterone Receptor and Myc in Endometrial Cancer", as cited below [1] and "Estrogen receptor alpha in the brain mediates tamoxifen-induced changes in physiology in mice" for immunohistochemistry knockout validation (figure 5a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting of ER alpha in Ishikawa, parental ECC1 cells and individual ESR1 KO ECC1 clones treated with 20 nM LBH589 for 24 hr. Beta-actin serves as a loading control, from [1].

Antibody: ERalpha

Catalog number: sc-8002

Summary: Mouse monoclonal IgG2a specific for an epitope mapping between amino acids 570-595 at the C-terminus of ERalpha of human origin. Recommended for detection of estrogen receptor alpha of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Company: Santa Cruz

Western blot

ESR1 KO ECC1 cells. Cells were solubilized in cold NP-40 cell lysis buffer (150 mM NaCl, 50 mM Tris/HCl, pH 7.4, 1% NP-40 with a protease and phosphatase inhibitor cocktail from Pierce)

HRP-conjugated secondary

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.