This is a knockout-validated antibody summary, based on the publication "One-step generation of conditional and reversible gene knockout", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Company: Bio-Techne
Antibody: ERR beta/NR3B2
Catalog number: PP-H6705-00
Summary: Mouse monoclonal IgG2a against human ERR beta. Reacts with human and cross-reacts with mouse and rat.
Suitable for western blot, immunohistochemistry (paraffin), immunoprecipitation and ELISA.
Western blot
WT and Esrrb KO ESCs. Cells were lysed in buffer containing complete protease-inhibitor cocktail tablets (11697498001, Roche).
1:1,000 dilution.
1:5,000 dilution horseradish peroxidase horse anti-mouse IgG (Cell Signaling).
ECL prime western blotting detection system (RPN2133, GE Healthcare).
Western blot confirming the loss of Esrrb protein expression following Cre recombination. Please see Supplementary Figure 6b in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- gene