ERRα antibody | knockout validation | Cell Signaling 13826

This is a knockout-validated antibody summary, based on the publication "MYC-dependent oxidative metabolism regulates osteoclastogenesis via nuclear receptor ERRα", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: ERRα

Catalog number: 13826

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human ERRα protein. Reacts with human, mouse, rat, and monkey. It is predicted to react based on 100% sequence homology with bovine, dog, pig, and horse. Suitable for western blot and Chromatin IP/IP-seq.

Validation Method

Western blot

Sample

ERRα+/+ and ERRα-/- human osteoclasts.

Figure

Immunoblot of the indicated proteins in whole-cell lysates of ERRα+/+ and ERRα-/- osteoclasts. α-tubulin served as a loading control. Please see Supplemental Figure 14c in the article [1].

References
  1. Bae S, Lee M, Mun S, Giannopoulou E, Yong Gonzalez V, Cross J, et al. MYC-dependent oxidative metabolism regulates osteoclastogenesis via nuclear receptor ERR?. J Clin Invest. 2017;127:2555-2568 pubmed publisher