ERCC8 antibody | knockout validation | Abcam ab137033

This is a knockout-validated antibody summary, based on the publication "Elements That Regulate the DNA Damage Response of Proteins Defective in Cockayne Syndrome", as cited below [1]. Another article "TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A" also indicates the knockout validation of this antibody for western blot (see figure 4c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: ERCC8

Catalog number: ab137033

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human ERCC8. Reacts with human by western blot, and immunoprecipitation. Unsuitable for flow cytometry, immunocytochemistry/immunofluorescence or immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

CSA-corrected (CSA(+)) or CSA-deficient (CSA(-)) CS3BE cells.

Secondary incubation

Secondary HRP-conjugated antibodies.

Detection

ECL prime (GE Healthcare Bio-Sciences, Pittsburgh, PA) or SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific Inc.) were used to visualize proteins on a ChemiDoc XRS+ system (Bio-Rad Laboratories, Hercules, CA).

Figure

Western blot of whole cell extracts of CSA protein and GAPDH (loading control) in CSA-corrected (CSA(+)) or CSA-deficient (CSA(-)) cells. Please see Figure 7a in the article [1].

References
  1. Iyama T, Wilson D. Elements That Regulate the DNA Damage Response of Proteins Defective in Cockayne Syndrome. J Mol Biol. 2016;428:62-78 pubmed publisher
  2. Pines A, Dijk M, Makowski M, Meulenbroek E, Vrouwe M, van der Weegen Y, et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun. 2018;9:1040 pubmed publisher