This is a knockout-validated antibody summary, based on the publication "Development and Characterization of Novel Rat Anti-mERβ Sera", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Santa Cruz Biotechnology
Antibody: ERα
Catalog number: sc-7201 (H-184)
Summary: Rabbit polyclonal antibody raised against resides 2-185 of human ERα. Supplier recommended for WB, IP, IF, IHC and elisa. Reacts with human, mouse and rat ERα.
Note :This antibody will be discontinued December 15, 2016.
Western blot
Lysates of mammary gland tissue from wild-type and ERα knockout mice.
TBS containing 5% milk powder and 0.05% Tween 20.
Anti-ERα overnight in blocking buffer.
HRP-conjugated goat anti-rabbit Ig G (172-1019, Bio-Rad) for 1 hour at room temperature.
ECL Prime (RPN 2109, GE Healthcare).
See Figure 2 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.