This is a knockout-validated antibody summary, based on the publication "The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure 1 in [1]. Wild-type and EPS8 knockout endothelial cells were stained with anti-EPS8 antibody in western blot.

Mouse monoclonal

Company: BD Bioscience

Antibody: EPS8

Catalog number: 610144

Summary: Mouse monoclonal antibody raised against residues 628-821 of mouse EPS8.

Supplier recommended for WB, IP, IF and ICC.

Reacts with human, mouse, rat and dog EPS8.

Western blot

Immunocytochemistry

Figure 2. From Figures 2 in [1]. Wild-type and EPS8 knockout endothelial cells were stained with anti-EPS8 antibody in immunocytochemistry.

Western blot: lysates of lung endothelial cells (ECs) from EPS8 knock out mice reconstituted with lentivirally expressed GFP or GFP-EPS8.

Immunocytochemistry: endothelial cells from wild-type and EPS8 knockout mice.

Western blot: not stated.

Immunocytochemistry: PBS containing 2% BSA.

Western blot: anti-EPS8 antibody.

Immunocytochemistry: anti-EPS8 antibody.

Western blot: HRP-conjugated secondary antibody.

Immunocytochemistry: not stated.

Western blot: ECL reagent (GE Healthcare).

Immunocytochemistry: Leica TCS confocal microscope.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.