This is a knockout-validated antibody summary, based on the publication "Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot showing loss of ENTPD3 protein in DRG and bladder collected from adult mice. Actin was used as loading control.

Sheep polyclonal

Company: R&D Systems

Antibody: ENTPD3

Catalog number: AF4464

Summary: Sheep polyclonal against Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L3/ENTPD3

Gln44-Pro485. Detects mouse CD39L3/ENTPD3 in western blots, immunoprecipitation and ELISA. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human CD39L3 is observed.

Western blot | Immunohistochemistry

WB: Male WT and Entpd3 -/- (3 month-old; ~25 g; n=3 for each genotype) DRG and bladder tissue was collected and digested in modified RIPA buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% SDS, 0.5% deoxycholate) supplemented with protease inhibitors (Roche Complete Mini).

IHC: Tissue sections from 3 WT and 3 Entpd3 -/- mice were stained immunohistochemically as previously reported (Taylor-Blake & Zylka, 2010).

WB: 0.2 μg/ml overnight at 4°C

IHC: skin, 1:75; DRG and spinal cord 1:400

WB: rabbit anti-sheep horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature (0.16 μg/ml; #31480, Thermo Scientific).

IHC: secondary antibodies conjugated with Alexa Fluor dyes were purchased from Invitrogen

IHC: Stained sections were imaged on a Zeiss LSM 510 confocal microscope or a Zeiss LSM 710 confocal microscope.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.