This is a knockout-validated antibody summary, based on the publication "A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of EMC3 in control and EMC3-knockout Neuro-2a cells. Actin served as loading control. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: EMC3

Catalog number: sc-365903

Summary: Mouse monoclonal IgG2a (kappa light chain) specific for an epitope mapping between amino acids 51-83 within an internal region of human TMEM111 (EMC3). Recommended for detection of TMEM111 of human, mouse, and rat by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, canine, bovine, porcine and avian.

Figure 2. Western blot of EMC3 in control and EMC3-knockout 293FT cells. Actin served as loading control. From [1].

Western blot

Control and CRISPR-mediated EMC3 knockout 293FT and Neuro-2a cells. Cells were lysed with RIPA buffer supplemented with protein inhibitor cocktail (Roche Life Science).

5% skim milk in TBST overnight.

4μg/ml for 2 hours.

HRP-conjugated secondary antibody for 1 hour.

ECL western blotting substrate (Thermo Scientific), according to the manufacturer’s instructions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.