This is a knockout-validated antibody summary, based on the publication "A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of EMC2 knockout sublines of 293FT cells. Actin served as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: EMC2

Catalog number: sc-166011

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-297 representing full length human TTC35 (EMC2). Recommended for detection of TTC35 of human, mouse, and rat by western blotting, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Control and CRISPR-mediated EMC2 knockout 293FT cells. Cells were lysed with RIPA buffer supplemented with protein inhibitor cocktail (Roche Life Science).

5% skim milk in TBST overnight.

1:200 dilution for 2 hours.

HRP-conjugated secondary antibody for 1 hour.

ECL western blotting substrate (Thermo Scientific), according to the manufacturer’s instructions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.