ELL2 antibody | knockout validation

ELL2 antibody | knockout validation | Santa Cruz sc-376611

This is a knockout-validated antibody summary, based on the publication "The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Verification of successful ELL2 CRISPR-Cas9 knockout in two L363 clones using Western blot with antibodies towards ELL2. From [1].

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: ELL2

Catalog number: sc-376611

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 377-461 mapping within an internal region of human ELL2. Recommended for detection of ELL2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and ELL2 knockout L363 cells. Cells were lysed using 2× Laemmli sample (100 μl) and 2-mercaptoethanol.

Primary incubation

Overnight.

Detection

Development reagent (Bio-Rad).

References

Ali M, Ajore R, Wihlborg A, Niroula A, Swaminathan B, Johnsson E, et al. The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression. Nat Commun. 2018;9:1649 pubmed publisher

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