EGFR antibody | knockout validation | Abcam ab52894

This is a knockout-validated antibody summary, based on the publication "Myeloid Cell-Derived HB-EGF Drives Tissue Recovery After Pancreatitis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

EGFR antibody | knockout validation | Abcam ab52894 figure 1
Figure 1. H&E staining and IHC for EGFR on pancreata of Egfrf/f;Ptf1aFlpO/+;R26CreERT/+ mice were harvested 1 day after vehicle/tamoxifen treatment after 2 weeks of cerulein. The boxes are the magnified area. Scale bar: 20 μm. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: EGFR

Catalog number: ab52894

Summary: Rabbit monoclonal IgG against a synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR. Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, flow cytometry, immunocytochemistry/immunofluorescence and immunohistochemistry (paraffin and frozen).

Validation Method

Immunohistochemistry

Sample

Control and EGFR-KO mice pancreas. Pancreata were removed and fixed in Z-fix (NC9050753; Anatech, Ltd, Battle Creek, MI) overnight.

Detection

Immunohistochemistry slides were scanned on a Pannoramic SCAN scanner (Perkin Elmer, Seattle, WA).

Clone note

The same clone (EP38Y) is sold as Abcam ab52894, ab174481; Invitrogen MA5-14485.

References
  1. Wen H, Gao S, Wang Y, Ray M, Magnuson M, Wright C, et al. Myeloid cell-derived HB-EGF Drives Tissue Recovery After Pancreatitis. Cell Mol Gastroenterol Hepatol. 2019;: pubmed publisher