E2F6 antibody | knockout validation | Kerafast EC0002

This is a knockout-validated antibody summary, based on the publication "E2F6 initiates stable epigenetic silencing of germline genes during embryonic development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

E2F6 antibody | knockout validation | Kerafast EC0002 figure 1
Figure 1. Western blotting of E2F6 in MEFs derived from WT and E2f6−/− embryos and MEFs CRISPR-Cas9 clones. β-ACTIN was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG

Company: Kerafast

Antibody: E2F6

Catalog number: EC0002

Summary: Mouse monoclonal antibody against full length human E2F6. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, ELISA and Gel Supershift Assay.

Validation Method

Western blot

Sample

WT and E2f6−/− KO MEFs.

Blocking agent

Tris Buffered Saline (TBS), 5% milk, 0.05% Tween for 1 h at room temperature.

Primary incubation

1:500 dilution.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies.

Detection

Chemiluminescence detection using the ECL detection reagent (Amersham, GE Healthcare).

References
  1. Dahlet T, Truss M, Frede U, Al Adhami H, Bardet A, Dumas M, et al. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nat Commun. 2021;12:3582 pubmed publisher