This is a knockout-validated antibody summary, based on the publication "Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: ThermoFisher

Antibody: E2F1

Catalog number: 32-1400

Summary: Mouse monoclonal IgG2a against recombinant human E2F-1 protein. Reacts with human, mouse and rat by western blot, immunoprecipitation, immunohistochemistry, and flow cytometry.

Western blot

WT and E2f1−/− mouse liver.

Western blot of protein expression in WT and E2f1−/− mouse liver. Please see Figure 5E in the article [1].

The same clone (KH95) is sold as Santa Cruz Biotechnology sc-251, sc-251 AC, sc-251 X; LifeSpan Biosciences LS-C95567, LS-C88541, LS-C88542, LS-C88543; Abcam ab4070; Invitrogen MA5-14341, MA5-14344, 32-1400.