This is a knockout-validated antibody summary, based on the publication "The RIP140 gene is a transcriptional target of E2F1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Adipocyte differentiation of mouse embryo fibroblasts in wild-type (WT) and E2F1−/− MEFs. The levels of E2F1 protein were detected by western-blot analysis and loading control was performed using an anti-TBP antibody. From [1].

Antibody: E2F1

Catalog number: sc-193

Summary: Rabbit polyclonal IgG raised against an epitope mapping at the C-terminus of E2F-1 of human origin. Recommended for detection of E2F-1 and, to a lesser extent, E2F-2 and E2F-3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA; also reactive with additional species, including equine, canine, bovine and porcine.

Company: Santa Cruz Biotechnology

Western blot

Wild-type (WT) and E2F1−/− MEFs isolated from 13.5 days-old embryos and differentiated into adipocytes.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.