This is a knockout-validated antibody summary, based on the publication "Neuronal nitric oxide synthase localizes to utrophin expressing intercalated discs and stabilizes their structural integrity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2b

Company: Developmental Studies Hybridoma Bank

Antibody: Dystrophin

Catalog number: Mandra1

Summary: Mouse monoclonal IgG2b against human dystrophin. Recommended for detection of human, mouse, fish, and Xenopus protein by western blot and immunofluorescence.

Immunohistochemistry

Heart tissue sections from WT, mdx (Dystrophin null), mdx:utr (Dystrophin null, Utrophin null), and nNOSμ KO mice. Tissue was dissected out and frozen in OCT for cryosectioning.

1:10 dilution.

Images were taken using a Nikon Eclipse T1 or Carl Zeiss AxioVert 200M microscope with a 40× or 20× objective and processed using NIS-Elements BR or Carl Zeiss AxioVision software.

Please see Figure 3a in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.