This is a knockout-validated antibody summary, based on the publication "WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of HEK293T WT, DVL TKO clone #4, DVL TKO clone #6, and LRP5/6 DKO cells treated with WNT3A CM for 9 hr. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Dvl-3

Catalog number: sc-8027

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 607-704 mapping at the C-terminus of mouse Dvl-3. Recommended for detection of Dvl-3 of mouse, rat and human origin by western blot and immunoprecipitation.

Western blot

HEK293T WT, DVL TKO, and LRP5/6 DKO cells.

1:1,000 dilution overnight at 4°C with rotation.

1:5,000 dilution (LiCOR Biosciences).

LiCOR Odyssey imager.