This is a knockout-validated antibody summary, based on the publication "Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Drp1 cytosol fractions from the indicated KO HeLa cell lines. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG2a

Company: BD Biosciences

Antibody: Drp1

Catalog number: 611739

Summary: Mouse monoclonal IgG2a against human Drp1 aa. 19-201. Reacts with human, mouse, rat, and dog. Suitable for western blot and immunofluorescence.

Western blot | Immunocytochemistry

Figure 2. Confocal images of WT and Drp1-KO HeLa cells lines; Drp1 (green) and Tom20 (red). Bar, 20 µm. Insets, magnified images of the boxed regions. Bar, 5 µm. Bottom right, fluorescence obtained for the Drp1 channel. Heat map, Drp1 fluorescence intensity (FI). From [1].

WT and Drp1-KO HeLa cells lines.

WB: PBS containing 0.3% nonfat dry milk and 0.1% Tween 20.

ICC: primary antibodies for 2 h.

WB: AP-conjugated secondary antibodies.

ICC: Alexa-conjugated secondary antibodies (Invitrogen) for 1 h.

WB: Immun-Star AP Western blotting detection reagents (Bio-Rad Laboratories) and LAS-1000 (FUJIFILM).

ICC: inverted structured illumination microscope (ApoTome with Axio Observer.Z1; Carl Zeiss) with a 63×/1.4 NA oil differential interference contrast Plan-Apochromat objective, a digital CCD camera (AxioCam MRm; Carl Zeiss), and Zeiss ZEN 2010 acquisition software.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.