This is a knockout-validated antibody summary, based on the publication "Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Total cell lysates prepared from the indicated HCT116 cell lines were analyzed by immunoblotting for Drp1 and actin (loading control). For comparison, different amounts of proteins (1:3 ratio) were applied. From [1].

Mouse monoclonal IgG1

Company: BD

Antibody: Drp1

Catalog number: 8/DLP1

Summary: Mouse monoclonal IgG1 against rat DLP1 (aa. 601-722). Reacts with rat, human, mouse, and dog. Suitable for western blot and immunofluorescence.

Western blot

WT and FIS1-KO, TBC1D15-KO, MFF-KO and DRP1-KO HCT116 cell lines. Cells were solubilized with 2% CHAPS buffer (25 mM HEPES-KOH pH 7.5, 300 mM NaCl, 2% (wt/vol) CHAPS, protease inhibitor cocktail (Roche, Indianapolis, IN).

Horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ).

ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.