This is a knockout-validated antibody summary, based on the publication "Noncanonical function of DGCR8 controls mESC exit from pluripotency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Drosha
Catalog number: 3364
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly953 of human Drosha. Reacts with human and mouse. Suitable for western blot and immunoprecipitation.
Western blot
WT and Drosha-KO murine embryonic stem cells (mESCs). Total cellular protein was extracted from 1 × 106 mESC pellets using a NP-40–based lysis buffer (1% NP-40, 137 mM NaCl, 20 mM Tris-HCl, and 1 mM EDTA) complemented with EDTA-free protease inhibitor cocktail (Roche).
rabbit IgG HRP-linked antibody 1:20,000 diluted (08/2012; Cell Signaling Technology)
Clarify Western ECL substrate (Bio-Rad Laboratories) kit and detected using an imaging system (ChemiDoc MP; Bio-Rad Laboratories).
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