This is a knockout-validated antibody summary, based on the publication "Tead and AP1 Coordinate Transcription and Motility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of Dock4 in HCT116 cells expressing Crispr knockout constructs against Dock4 and Dock9 (Dock4/9 KO). GAPDH was used as loading control. From [1].

Rabbit polyclonal IgG

Company: Bethyl

Antibody: Dock4

Catalog number: A302-263A

Summary: Rabbit polyclonal IgG against an epitope mapping between aa 1725 and 1775 of human DOCK4. Reacts with human protein by western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence.

Western blot

Control and Dock4/9-KO HCT116 cells.

HRP conjugated secondary antibodies (Jackson Laboratories).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.