This is a knockout-validated antibody summary, based on the publication "Dnmt3b Deficiency in Myf5 +-Brown Fat Precursor Cells Promotes Obesity in Female Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 κ
Company: Santa Cruz
Antibody: Dnmt3b
Catalog number: sc-393845
Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 27-52 near the N-terminus of human Dnmt3b. Recommended for detection of Dnmt3b of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and Dnmt3b KO mouse interscapular brown adipose tissue.
Alexa Fluor 680-conjugated secondary antibodies (ThermoFisher Scientific).
Li-COR Imager System (Li-COR Biosciences, Lincoln, NE, USA).
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