This is a knockout-validated antibody summary, based on the publication "H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Dnmt3a
Catalog number: ab188470
Summary: Rabbit monoclonal IgG against a synthetic peptide within human Dnmt3a (aa 600-700). Reacts with mouse, rat and human by western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.
Western blot
Parental and Dnmt3a-KO mouse embryonic stem cells (mESC).
5% non-fat milk in PBS plus 0.5% Tween-20.
Horseradish peroxidase-conjugated anti-rabbit secondary antibodies (GE Healthcare).
Immunoblots of lysates generated from parental and sgDnmt3a mESCs ectopically expressing HA-tagged DNMT3A2. Vinculin was used as a loading control. Endogenous expression of the long isoform (DNMT3A1) and short isoform (DNMT3A2) are indicated. Please see Extended Data Figure 3a in the article [1].
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