This is a knockout-validated antibody summary, based on the publication "Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
mouse monoclonal IgG1 κ
Company: Santa Cruz
Antibody: Dnmt2
Catalog number: sc-365001
Summary: Mouse monoclonal antibody against amino acids 121-391 of human Dnmt2 (TRDMT1). Recommended for detection of Dnmt2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and TRDMT1-KO 293 cells.
5% non-fat milk in PBS.
1:400 dilution at 4°C overnight.
At room temperature for 1 h.
Chemiluminescent HRP substrate was purchased from Millipore (Catalog#: WBKLS0500). Images were taken in the BIO-RAD Universal Hood II machine with corresponding ImageLab software.
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