This is a knockout-validated antibody summary, based on the publication "Dickkopf-1 promotes Vascular Smooth Muscle Cell proliferation and migration through upregulating UHRF1 during Cyclic Stretch application", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of the DKK1 protein levels in the aortas from the CTR and Dkk1SMKO mice (n=3). From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Dkk-1

Catalog number: sc-374574

Summary: Mouse monoclonal antibody against amino acids 1-120 of human Dkk-1. Recommended for detection of Dkk-1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Control and Dkk1-smooth muscle KO mice.

5% nonfat dry milk.

1:50 dilution overnight at 4 °C.

HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories) for 1 h at room temperature.

Immobilon ECL substrate (Millipore), and the blots were imaged with a ChemiDoc Touch Imaging System (Bio-Rad).