This is a knockout-validated antibody summary, based on the publication "Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (a) Frozen sections of 6 month-old KC;Dectin-1+/+ and KC;Dectin-1–/– pancreata were co-stained for Dectin-1 and CD45 or (b) Dectin-1 and CK19 and imaged by confocal microscopy. Representative images are shown (scale bar = 50µm). (c) Frozen sections of orthotopic KPC-derived pancreatic tumors were co-stained for Dectin-1 and CD45 or (d) Dectin-1 and CK19 and imaged by confocal microscopy. Representative images are shown (scale bar = 50µm). Please see Suppl. Figure 1a-d in the article [1].

Rat monoclonal IgG2b

Company: Abcam

Antibody: Dectin-1

Catalog number: ab53427

Summary: Rat monoclonal IgG2b against Dectin-1. Reacts with mouse by immunoprecipitation, immunohistochemistry (frozen) and flow cytometry. Not suitable for immunohistochemistry (paraffin) or immunohistochemistry (resin).

Immunohistochemistry

Frozen sections of pancreata and pancreatic tumors from Dectin-1+/+ and Dectin-1–/– mice.

20 μg/ml.

Immunofluorescent images were acquired using the Zeiss LSM700 confocal microscope with ZEN 2010 software (Carl Zeiss, Thornwood, New York).

The same clone (2A11) is sold as Bio-Rad MCA2289F, MCA2289PE, MCA2289FB, MCA2289A647, MCA2289B, MCA2289EL, MCA2289, MCA2289GA, MCA2289FA, MCA2289T, MCA2289PET, MCA2289FT, MCA2289A647T, MCA2289BT; LifeSpan Biosciences LS-C188260, LS-C188261, LS-C188262, LS-C188263, LS-C188264, LS-C188265, LS-C188266; Abcam ab21646, ab53427; Invitrogen MA5-16477, MA5-16478, MA5-16479, MA5-16480, MA5-16481, MA5-16482, MA5-16483; Hycult Biotech HM1067.