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This is a knockout-validated antibody summary, based on the publication "Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. DAPK1 deficiency does not affect expressions of FADD, RIPK1, RIPK3, or MLKL in BMDMs. From [1].

Mouse monoclonal IgG1

Company: Sigma

Antibody: Death Associated Protein Kinase

Catalog number: D2178

Summary: Mouse monoclonal IgG1 against recombinant human DAP kinase. Reacts with human and monkey. Suitable for western blot, immunoprecipitation and microarray.

Western blot

WT and DAPK1-KO mouse bone marrow-derived macrophages (BMDMs).

5% non-fat milk and 0.1% Tween-20 in a TBST buffer of 50 mM Tris-HCl pH 7.4 and 150 mM NaCl) at room temperature for 30 min,

1:1,000 dilution at 4 °C overnight.

Horseradish peroxidase-conjugated secondary antibodies in blocking buffer at room temperature for 1 h.

ECL Western blot detection reagents (Advansta, K-12045-D50), with signals detected by X-ray film (Fujifilm).