This is a knockout-validated antibody summary, based on the publication "Endocytic adaptors Arh and Dab2 control homeostasis of circulatory cholesterol", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: BD Transduction

Antibody: Dab2

Catalog number: 610465

Summary: Mouse monoclonal IgG1 against mouse p96 (Dab2) aa. 31-45. Reacts with mouse, human and rat. Suitable for western blot and immunofluorescence. Not recommended for immunoprecipitation or immunohistochemistry.

Western blot

Cells were lysed in RIPA buffer (20 mM Tris pH 7.5, 50 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate).

1/5,000 dilution.

1/5,000 dilution HRP-conjugated anti-mouse secondary antibodies (BioRad) following the instructions from the manufacturer.

Chemiluminescence detection using SuperSignal West Extended Duration Substrate (Pierce Biotech).

Please see Figure 2b,c in the article [1].

Antibody used could be anti-Dab2 rabbit polyclonal (TMD) antibody (1 in 1,000). The authors have been contacted, and no reply has been received yet.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.