DRP1 antibody | knockout validation | BD Biosciences 611738
DOI
//dx.doi.org/10.13070/ko.en.6.1681
Date
2016-10-04

This is a knockout-validated antibody summary, based on the publication "Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DRP1 antibody | knockout validation | BD Biosciences 611738 figure 1
Figure 1. Immunoblots of DRP1 in cell lysates of mouse macrophages derived from WT or Drp1fl/fl-LysMCre mice. Caspase-1 was used as loading control. From [1].
Antibody information

Antibody: DRP1

Catalog number: 611738

Summary: Mouse IgG2a raised against human Drp1 aa. 19-201. Detects human, mouse, rat, and dog proteins in western blot and immunofluorescence.

Company: BD Biosciences

Validation Method

Western blot

Sample

macrophages derived from WT or Drp1fl/fl-LysMCre mice.

Primary incubation

1/1,000–1/2,000 dilution

Note:

Caspase-1 might not be a good housekeeping gene to use as a loading control, but authors conclude that shows no change, thus it is valid.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Kang S, Fernandes-Alnemri T, Rogers C, Mayes L, Wang Y, Dillon C, et al. Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3. Nat Commun. 2015;6:7515 pubmed publisher