DRG2 antibody | knockout validation | Sigma-Aldrich GW22260A
DOI
//dx.doi.org/10.13070/ko.en.6.1720
Date
2016-10-07

This is a knockout-validated antibody summary, based on the publication "Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DRG2 antibody | knockout validation | Sigma-Aldrich GW22260A figure 1
Figure 1. Western blot of DRG2 protein levels in MEF cells derived from wild-type (+/+), DRG2-KO heterozygous (+/–), and DRG2-KO homozygous (–/–) mice. Actin served as loading control. From [1].
Antibody information

Chicken polyclonal

Company: Sigma-Aldrich

Antibody: DRG2

Catalog number: GW22260A

Summary: Chicken polyclonal against recombinant developmentally regulated GTP binding protein 2. sequence 1-64. Reacts with human and mouse in western blot.

Validation Method

Western blot

Sample

MEF cells derived from wild-type (+/+), heterozygous (+/–), and homozygous (–/–) mice

Detection

Immunoreactivity was detected using a GE HealthCare Image Quant system

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Mani M, Lee U, Yoon N, Kim H, Ko M, Seol W, et al. Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling. Mol Biol Cell. 2016;27:334-48 pubmed publisher